Therefore, to investigate the relationship between the PACAP expression and migraineurs’ characteristics, the migraine group was subdivided into male and female groups, interictal and attack groups, and with aura (MA) and without aura (MO) groups, but no significant differences in PACAP levels were observed (p > 0.05) (Figs. 3, 4, 5). The gene discussed is ADCYAP1; the disease is migraine disorder.