MKI67 and in situ carcinoma: P16/Ki67 dual staining is used as a tool for CIN2 triage; however, Wentzensen et al. reported that P16/Ki67 dual staining yielded a 78.9 % positive rate for CIN2 and an 86.9 % positive rate for CIN3/CIS/cancer but also exhibited a 41.1 % positive rate for the CIN2− group [15].