Some researchers also investigated possible apoptotic mechanisms induced by Cinobufacini in HCC cell lines HepG(2) and Bel-7402 and the results suggested that Cinobufacini could induce apoptosis of HCC cells through mitochondria- and Fas-mediated caspase-dependent pathways with the increase of treatment time [19]. This evidence concerns the gene FAS and hepatocellular carcinoma.