In summary, three effects with increasing passage number were seen: (i) no influence, either for absolute values or for inducibility (cbfa1, and oct-4 expression); (ii) the decrease of absolute values in OM/D and BM with maintained inducibility (TNAP activity, and pthr expression excepted P10), and (iii) the decrease of absolute values in OM/D and or BM as well as alteration of inducibility (bglap and pparγ expression). This evidence concerns the gene RUNX2 and ocular melanoma.