In this study, we demonstrate that imatinib and nilotinib treatment of glioblastoma and patient-derived glioblastoma stem cells results in increased tyrosine phosphorylation of several signalling proteins centrally important for cell motility including p130Cas, focal adhesion kinase (FAK), and paxillin (PXN), and strikingly increases tumour cell and stem cell migration and invasion. This evidence concerns the gene PXN and glioblastoma.