To further examine the functional role of TEAD4 splicing switch, we applied a recently developed approach, engineered splicing factors (ESFs)39, 40, 41, 42, to specifically manipulate TEAD4 splicing and to test whether the splicing changes of TEAD4 can directly affect YAP-mediated EMT and tumour proliferation. This evidence concerns the gene TEAD4 and neoplasm.