Fig. 5c top panel shows the number of size-classified metastatic nodules that formed in mice injected with Luc control or Trpv4 knocked-down 4T1 cells. The results showed that silencing of Trpv4 expression reduced the number of lung metastasis by approximately 80% (60 versus 12 nodules). There was no statistically significant difference in the size of the nodules formed (Fig. 5c, bottom panels). This is consistent with the earlier observation that TRPV4 does not affect cancer growth/proliferation. A complete list of nodules counting and IHC results are provided in Supplementary Table 3. The gene discussed is TRPV4; the disease is cancer.