Wild type p53-expressing U2Os and p53-deficient Saos-2 osteosarcoma cells were treated with 5 and 35 nm sized AgNPs in various concentrations (5–100 μM) for 24 or 48 h and cell viability was determined by MTT assay (Fig. 1c–f). This evidence concerns the gene TP53 and osteosarcoma.