In this study, the roles of O-GlcNAcylation in CCA were examined in two CCA cell lines, KKU-213 and KKU-214 using the specific siRNA to OGT (GCGUGUUCCCAAUAGUGUAtt) or OGA (GUCCACAGAUGGCUCUAAAtt)15. Here, OGA is linked to cholangiocarcinoma.