Among the different PD-L1 monoclonal antibodies, PD-L1 (E1L3N) showed the highest expression rate in cancer cells (14.7, 14.7, 11.0, 2.3 %) and immune cells (37.6 %) and PD-L1 (SP142) showed the lowest expression rate in cancer cells (11.5, 11.0, 6.9, 0.5 %) and immune cells (19.3 %) for all cut-off values (1, 5, 10, and 50 %) (Table 2; Fig. 1). The gene discussed is CD274; the disease is cancer.