In order to understand whether such signaling pathway contributes to the effect of UCN-01 on CRC-SCs, we evaluated the expression levels of total and phosphorylated PDK1 (pS241), PKCα/β II (pT638/41), CDC25C (pS216) and Chk1 (pS345) in two representative CRC-SC lines. This evidence concerns the gene PDK1 and colorectal carcinoma.