We injected the four groups of mice with B16F1 melanoma cells transfected with pRK5 empty vector plasmids, pRK5-FADD plasmids, pRK5-FADD plasmids plus miR-7a mimic, or pRK5-FADD plasmids plus miR-7a inhibitor through tail vein. This evidence concerns the gene FADD and melanoma.