Furthermore, we demonstrated that activation of the Wnt/β-catenin signaling in HCT116 cells resulted in reduction of E-cadherin and ZO-1, which is in agreement with previous studies that the Wnt/β-catenin pathway contributed to EMT, migration and invasion of cells, [5, 8, 9, 28] suggesting that Wnt/β-catenin signaling may contribute to the development of cancers depending on the coordinated regulation between its downstream non-coding RNA and protein coding genes. The gene discussed is TJP1; the disease is cancer.