LEF1 and cancer: As activation of Wnt pathway is reported to promote EMT in cancer cells, [5, 8, 9, 28] we confirmed this by activating Wnt pathway using LiCl treatment or LEF1 overexpression in HCT116 cells and detected decreased expression of epithelial markers E-cadherin and ZO-1 (Supplementary Figure S3E and S3F).