As we found that high levels of HULC expression were strongly associated with increasing metastasis in HCC, we used western blot (Figure 4A) and immunohistochemistry (Figure 4B) to analyze EMT markers, including E-Cadherin, N-Cadherin, ZO-1, Vimentin, β-Catenin, Snail and ZEB1, to compare EMT features between HULC(L) and HULC(H) within our clinical HCC samples. This evidence concerns the gene VIM and hepatocellular carcinoma.