Furthermore, 5-FU cross-resistant PacR cancer cells display increased EMT associated with P-gp, which can be repressed by transient silencing of TUBB3. Notably, the knockdown of TUBB3 resulted in the attenuation of PacR/5-FU and PacR/CIS cancer cell-induced tumors in vivo associated with regulated levels of Akt and VEGF. This evidence concerns the gene PGP and in situ carcinoma.