Expression of pluripotency and seminoma key factors was not significantly different between parental TCam-2 and TCam-2-ΔSOX2 clones, suggesting that a CRISPR/Cas9-mediated depletion of SOX2 does not lead to off-target effects, impinging on the undifferentiated and seminoma-like nature of TCam-2 cells (Figure S1C, S1D). The gene discussed is SOX2; the disease is seminoma.