We also found multinucleated cells within the differentiated subpopulation of in vivo grown TCam-2-ΔSOX2 cells as well as upregulation of the trophoblast stem cell marker EOMES. So, the OCT3/4/SOX17/TFAP2C negative subpopulation cleary recapitulates the in vitro differentiation process of TCam-2 cells into a mixed non-seminoma in vivo. This evidence concerns the gene TFAP2C and seminoma.