In this study, to examine the effects of PA-MSHA we selected three different NSCLC cell lines based on their different gene-expression status: A549 is an EGFR wild type cell line with primary EGFR-TKI resistance, PC-9 is an EGFR-TKI-sensitive cell line with an exon 19 deletion mutation, and NCI-H1975 is an acquired EGFR-TKI-resistant cell line with T790M and L858R mutations. The gene discussed is EGFR; the disease is non-small cell lung carcinoma.