ANXA5 and non-small cell lung carcinoma: To determine whether PA-MSHA can enhance the effects of Gefitinib on NSCLC cell apoptosis, cells were treated for 12 hours with PA-MSHA, Gefitinib, or Gefitinib in combination with increasing doses of PA-MSHA and then were stained with annexin V/PI and analyzed by flow cytometry (Figure 3A and 3B).