ALPK1 and cancer: Firstly, we successfully transfected a siRNA and ALPK1 vector into Lovo and A549 cancer cells so that the mRNA expression of ALPK1 could be reduced in the ALPK1 knockdown cancer cells, while the expression of ALPK1 would be accelerated in the cancer cells that already over-express it compared to the mock group (Fig. 6a).