In an effort to identify the downstream mechanisms by which PDGF induces bone cancer pain, we used WB to evaluate the activation (phosphorylation) of PDGFR, ERK and AKT by measuring levels of pPDGFRα/β, pERK and pAKT 14 days after treatment with PDGF siRNA. The gene discussed is EIF2AK3; the disease is bone cancer.