ING3 and breast carcinoma: To determine whether the association with H3K4me3 is required for ING3 to induce apoptosis, human mammary carcinoma MCF7 cells were transduced with the ING3WT, ING3Y362A, or ING3W385A proteins (which correspond to residues Tyr-8 and Trp-31 highlighted in the time-averaged structure of the ING3PHD-H3K4me3 complex from the MD simulation, Fig. 3D), and DNA damage was induced by treating the cells with doxorubicin.