In one of the first clinical trial reports of clinical outcome of radiation therapy followed by ipilimumab, the combination resulted in 18% of patients demonstrating a partial response by RECIST criteria [32], which is not significantly different from the reported response of ipilimumab alone.[33] Our data were obtained using a relatively immunogenic tumor with high baseline Treg infiltrate which may influence ideal timing of anti-CTLA4 blockade. This evidence concerns the gene CTLA4 and neoplasm.