Surprisingly, in contrast to the qRT-PCR data, HNF1β protein expression was high in poorly differentiated ICC (Fig 5Q–5R and 5Q'–5R') and, low and mislocated, i. e. non-nuclear, in well differentiated tumors (Fig 5M–5N and 5M'–5N'); moderately differentiated tumors showed intermediate expression levels (Fig 5O–5P and 5O'–5P'). This evidence concerns the gene HNF1B and intrahepatic cholangiocarcinoma.