Next, we detected the protein expression of VEGF-A in two GC cell lines (Fig 5A and 5B), VEGF-A was greatly increased by MACC1 upregulation (P < 0.001 and P = 0.008), but was decreased by shMACC1 (P = 0.039 and P = 0.041). This evidence concerns the gene VEGFA and gastric cancer.