In addition, IHC staining in subcutaneous tumors with GC xenografts showed that TWIST1 and VEGF-A were significantly greater for oxMACC1 cells versus vector-controls, and was markedly decreased for shMACC1 cells versus scramble-controls (P = 0.003 and P = 0.026, Figs 4C and 5E). This evidence concerns the gene TWIST1 and gastric cancer.