In addition, to strengthen our conclusion, additional studies need to be performed, such as treating the OVX rats with Wnt3a to determine whether it can prevent OVX-induced disc degeneration and treating the OVX rats with Wnt/β-catenin pathway inhibitor XAV-939 to determine whether it can reduce the therapeutic effect of oestrogen or PTH. Here, WNT3A is linked to intervertebral disk degenerative disorder.