Compared with the OVX group, mRNA and protein levels of Aggrecan and type II collagen in the OVX+E2 and OVX+PTH groups were higher, and expression of type I collagen, MMP-3 and MMP-9 were lower, showing that oestrogen and PTH could improve disc matrix synthesis and inhibit enzymes that can degrade extracellular matrix to reverse disc degeneration caused by ovariectomy. Here, PTH is linked to intervertebral disk degenerative disorder.