In our study, we firstly show that miR-320a promotes 5-FU resistance through raising EMT transformation in pancreatic cancer cells, exhibiting as down-regulation of epithelial cells markers (β-cadherin and E-cadherin) and up-regulation of mesenchymal cells markers (Fibronectin, N-cadherin, Vimentin, ZEB1 and Snail2). Here, ZEB1 is linked to familial pancreatic carcinoma.