Moreover, we clarified the role of autophagy, IGF-1, and lipid peroxidation products such as malondialdehyde (MDA) and 4-Hydroxy-2-Nonenal (4-HNE), which are associated with TLR7, at both the cellular and tissue levels under NAFLD. The gene discussed is TLR7; the disease is metabolic dysfunction-associated steatotic liver disease.