Both combined NS1 Ag Strip/IgM ELISA tests and combined qRT-PCR/IgM ELISA tests showed significant increases (p < 0.01) in positive detections in comparison to the use of IgM ELISA alone, which had positive detections of only 41.2% of primary dengue samples and 90.5% of secondary dengue samples. Here, CD40LG is linked to dengue disease.