The usage of two independent promoters for adipose‐specific gene disruption supports our conclusion that the primary defect underlying the whole‐body glucose intolerance and insulin resistance in PIKfyvefl/fl,aP2‐Cre+ and PIKfyvefl/fl,Aq‐Cre+ mice is the PIKfyve deficiency specifically targeted in adipose tissue. This evidence concerns the gene PIKFYVE and Insulin resistance.