Molecular tests showed the patients to be negative for deletional α-thalassaemia: -α4.2, –SEA (NG_000006.1:g.26264_45564del19301), –FIL (NG_000006.1:g.11684_43534), –THAI (NG_000006.1:g.10664_44164del33501), and the nondeletional α-thalassaemia mutations: initiation codon (HBA2:c.2delT), codon 30 (HBA2:c.91_93delGAG), codon 35 (HBA2:c.106T>C) and HbQS. Here, HBA2 is linked to thalassemia.