Based on the correlation analysis of miRNA and mRNA expression, the significance levels of miRNA and mRNA microarray data and previous literature, four miRNAs and 15 mRNAs, including MAML1, NNAT, VEZF1, ITGB3BP, SOX11, EZH2, EFNB1, CBX2, SMARCC1, PCP4, C2CD2, APP, MX1, PDXK and AGPAT3 were chosen to confirm their altered expression in DS fetal compared to controls by real-time PCR. The gene discussed is ITGB3BP; the disease is Dravet syndrome.