To investigate whether p53 regulates the promoter activity of PC via this potential p53 RE, we cloned the promoter region containing the p53 RE and the distal promoter (P2, between −92,280 and −91,186 bp) (which is responsible for glucose-mediated PC expression in pancreatic β-cells22) of the PC gene into the luciferase reporter vector pGL3 (Fig. 8c). Here, TP53 is linked to pachyonychia congenita.