BDNF and Alzheimer disease: To investigate the role of BDNF in the therapeutic potential of NSCs-based therapy for AD, we first cultured NSCs/progenitors (collectively called NSCs) and expanded their number from the hippocampi of postnatal day 1 of green fluorescent protein (GFP) expressing transgenic mice (please see Supplementary Fig. S1), and then established BDNF overexpressing NSCs by electroporation of BDNF construct and antibiotic selection.