NFKB1 and acute myeloid leukemia: To investigate if MUC1-C activates the DNMT1 promoter in AML cells, we transfected THP-1/CshRNA and THP-1/MUC1shRNA cells with a pDNMT1-Luc reporter that contains a consensus NF-κB binding site (GGGGTATCCC) at positions −833 to −824 upstream of the transcription start site (Figure 4A).