GPC3 and neoplasm: However, there was significant association between lower levels of GPC3 and higher number of distant metastases (Figure 2B, P=0.039), depth of invasion (Figure 2C, P=0.019), and tumor spread to the lymph nodes (Figure 2D, P=0.015) (n=51, 31 cases of Beijing cohort, 20 cases of Wuhan cohort).