EZH2 and glioblastoma: To examine the possible effect of EZH2 on mitochondrial activity in glioblastoma cells, we added oligomycin, pharmacological inhibitor of electron transport, and FCCP, a mitochondrial uncoupler, to transduced U251 (Figure 2A) and T98G (Supplementary Figure 1D) cells and then determined their oxygen consumption rates (OCRs) using an extracellular flux analyzer.