The Elispot assay revealed that the number of B16-F10 melanoma cell-stimulated IFN-γ secreting splenocytes was significantly increased in mice that were treated with HVJ-E+poly I:C (36.2±7) compared with HVJ-E (17.2±9.2) and poly I:C (21.1±3.8) treatments (Figure 2B). The gene discussed is IFNG; the disease is melanoma.