Upon stimulating the CAR RNA- or Blina-RNA-electroporated T cells that were generated by REP or CD3/CD28 bead stimulation, we found that the REP-expanded T cells showed enhanced anti-tumor activities for both the RNA CAR and Blina-RNA T cells, as evidenced by increased CD137 expression (Figure 4b) and increased lytic ability (Figure 4c), particularly when the RNA input dose was limited. This evidence concerns the gene CD28 and neoplasm.