Two colon cancer cell lines, HT29 and HCT116, respectively, derived from an adenocarcinoma and from a carcinoma (Table 1) and the fetal colon cell line CCD841CoN were analyzed by Western blot to determine to the levels of O-GlcNAcylation, OGT, OGA, and Glutamine:Fructose-6-P amidotransferase (GFAT), the rate-limiting enzyme of the HBP (Figures 1A,B). This evidence concerns the gene OGT and malignant colon neoplasm.