Sequencing results yielded a p.D32V (c.363 A > T) mutation and a TP53 ivs8-1 (c.783-1 G > A) splice site mutation in tumor region A. Comparing mutated allele frequencies, CTNNB1 and TP53 gene copies showed a similar range of both frequencies in area A1-3 (Fig. 1). This evidence concerns the gene CTNNB1 and neoplasm.