TBK1 and cardiac hypertrophy: To evaluate the necessity of TBK1 in SIKE-regulated cardiac hypertrophy, we generated a conditional Tbk1-knockout mouse line and crossed this line with an α-MHC promoter-driven Cre TG strain to obtain a mouse line with cardiac-specific ablation of Tbk1 (Tbk1-CKO; Supplementary Fig. 6a).