After confirming the participation of TBK1 and downstream AKT signalling in the progression of pathological cardiac hypertrophy, we infected NRCMs with AdshSike alone or in combination with AdshTbk1, followed by the treatment of Ang II for 48 h to examine the requirement of Tbk1 in the regulatory effects of Sike on cardiac remodelling. The gene discussed is SIKE1; the disease is cardiac hypertrophy.