To exclude the possibility that these effects of Traf6 were specific to pressure overload-induced cardiac hypertrophy, we next investigated whether Traf6 exacerbated Ang II-induced cardiac hypertrophy in vivo. As shown in Supplementary Fig. 3, 4 weeks of Ang II infusion in wild-type (WT) mice led to significantly increased HW/BW, LW/BW and HW/TL ratios compared with saline controls, as well as impaired contractile function and enlarged myocytes. This evidence concerns the gene TRAF6 and cardiac hypertrophy.