MCOLN2 and glioma: Moreover, blots of plasma membrane (ME) and cytoplasm (CE) fraction (Figure 4D) and cytofluorimetric analysis of unpermeabilized and permeabilized glioma cells demonstrated that TRPML-2 localization was both intracytoplasmic and in the plasma membrane, with highest expression in the cytosol (Supplementary Figure 1).