This allows the cell to rapidly adapt a distinct surface phenotype and to generate soluble mediators that can act on other cells.[19, 20] To analyze whether GARP can be shed from Treg and melanoma cells and thus act as paracrine factor for the induction of peripheral tolerance, the supernatants of activated versus resting Treg were analyzed by western blot and ELISA (Figure 3A). Here, LRRC32 is linked to melanoma.