For the evaluation of the copy number of other regions of 12p, utilizing probes targeting the ETNK1 gene at 12p12.1 and the DNM1L gene at 12p11.21, the ddPCR results were compared to that of ING4 gene at 12p13.31 using two PKS samples as well as the control sample. The gene discussed is ING4; the disease is tetrasomy 12p.