To investigate the relationship of hypoxia conditions with autophagy and mTOR pathway activity, Calu-6 tumors were analyzed for CAIX expression to annotate tumor hypoxic and normoxic regions and for levels of pS6 and LC3B to measure mTOR and LC3B pathway activities, respectively, using a dual IHC stain on a single slide. Here, MAP1LC3B is linked to neoplasm.