Then, we isolated CRC cells and tumor infiltrating lymphocytes (TIL) from 10 CRC patients tissues to assess the Granzyme B, IFN-γ, EBI3, IL-27p28, and IL-12p35 production and proliferation of TILs, Tregs after coculture with autologous CRC cells blocked by EBI3 blocking peptide. The gene discussed is EBI3; the disease is colorectal carcinoma.