In order to explore the mechanism of EBI3 in promoting CRC progression, a EBI3 blocking peptide (named EBI3 Bp), which showed the natural EBI3 signal blocking effect, whether in IL-35-mediated T cell proliferation restricted (Figure 2(a)) or IL-27-mediated Th1 cells differentiation (Figure 2(b)), was used to evaluate the role of EBI3 in tumor microenvironment. This evidence concerns the gene IL27 and colorectal carcinoma.