Thirteen subunits of respiratory complex I were down-regulated in HSP60-KN-293T cells (Figure 4(A)) as confirmed by western blotting of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 and 5 (NDUFA4 and NDUFA5) in HSP60-KN-293T cells (Figure 4(E)), suggesting that disruption of respiratory complexes in ccRCC was caused by HSP60 silencing. The gene discussed is HSPD1; the disease is nonpapillary renal cell carcinoma.