CD4 and infection: Depletion of Tregs by using anti-CD25 mAb showed that Tregs negatively affect efficient parasite control, whereas adoptive transfer of highly enriched CD4+ CD25+ Foxp3+ T cells resulted in increased peak parasitemia and production of disease exacerbating inflammatory cytokines like IFN-γ and IL-6 during early infection in the resistant C57BL/6 mice (40, 41).