The RT-qPCR analysis revealed that the RIG-I, MDA5 and their regulators such as RING finger protein 125 (RNF125), Interferon-stimulated gene 15 (ISG15), DExH (Asp-Glu-X-His) box polypeptide 58 (Dhx58, also known as LGP2) were up-regulated following CVS-11 and HEP-Flury infections (Figure 5A). This evidence concerns the gene ISG15 and infection.