In neuroblastoma cell lines, treatment with demethylating agent 5′aza-2-deoxycitidine and/or histone deacetylase inhibitor trichostatin A decreased the percentage of methylated cytosines in this specific region of CASR gene promoter and concomitantly restored CaSR expression in MYCN-amplified cell lines. The gene discussed is MYCN; the disease is neuroblastoma.